Suitability of different primers for specific molecular detection of Monilinia spp.
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چکیده
منابع مشابه
Specific PCR-primers for detection of Picoa lefebvrei desert truffle in Carex stenophylla roots
Picoa species are hypogeous desert truffles, which can be found in semi-arid ranges of North Africa, West Asian, South of Europe and Middle East, including Iran (Moreno et al. 2000, Jamali & Banihashemi 2012, 2013). Picoa species form symbiosis mainly on roots of annual and perennial herbaceous plants of the Helianthemum, including H. ledifolium and H. salicifolium var. salicifolium (Jamali & B...
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Specific Primers For Identification and Detection of Three Major Desert Truffle Species in Iran with Special Reference to Terfezia claveryi
ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for ...
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Mutual relationship between endophytic fungi and some gramineuos plants is one of the recently known host-microbe interactions. Endophytic fungi are classified in class Ascomycetes, order Hypocreales, tribe Balansieae and mostly genus Neotyphodium. They are transferred into the progeny via maternal stock and have many benificial effects to their host plants, such as resistance to pests and dise...
متن کاملExploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes
The efficacy of six different sets of primers targeted against 16S rRNA and virulence genes such as 'iap', 'hly' and 'prf' was evaluated in separate PCR assays. The primer pairs targeted against 16S rRNA resulted into amplification of 1.2 kb PCR product. However, sets of primers targeted against different regions of 'iap' produced 371 and 660 bp PCR products, respectively. The primer pair targe...
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ژورنال
عنوان ژورنال: Journal of Agricultural Sciences, Belgrade
سال: 2017
ISSN: 1450-8109,2406-0968
DOI: 10.2298/jas1702167d